Antigen-specific electrophoretic cell separation for immunological investigations

Preincubation of human blood lymphocytes with cell surface antigen specific antibodies under non-capping conditions reduces the electrophoretic mobility of the corresponding lymphocyte subpopulation. Antigen-positive and antigen-negative cells can be separated by free flow electrophoresis with high yield, purity and viability. The use of fluorescence-labelled second antibodies augments the induced decrease in net surface charge density, and allows rapid detection of antigen-positive cells in the fractions of electrophoresis. Carrier-free cell electrophoresis of human peripheral blood lymphocytes after reaction with anti-IgM-antibody or the monoclonal antibodies OKT4 or OKT8, and sandwich staining with tetrarhodamine isothiocyanate-labelled anti-IgG resulted in the large-scale separation of high pure human B and T lymphocyte subpopulations. Their functional integrity was shown in assays of lymphocyte transformation and of antigen-specific induction and regulation of antibody synthesis in vitro. These separate lymphocyte subpopulations are useful tools for immunological investigations. While, for instance, the effects of drugs on human lymphocytes are obscured by coincident changes in cell composition of the peripheral blood tested that do not by themselves reflect whole body immunocompetence, the cell separation and in vitro assays at a defined cell number and cell composition allow the recording of quantitative changes in the function of different cell subpopulations. We studied the influence of the anesthetic thiopental on separated human lymphocyte subsets. In both polyclonal lectin stimulation and in vitro antibody production, thiopental exhibited a noncytotoxic suppression of lymphocyte functions. B-Cells, T-helper and T-suppressor cells were equally affected and showed the same dose response.(ABSTRACT TRUNCATED AT 250 WORDS

Changing the way we learn: towards agile learning and co-operation

This paper addresses the need for learning and competence development in industrial organizations. The people that enter professional organizations today are part of a gamer generation that have some or much experience with on-line games. Therefore they are more open to e-learning and in general more open to access anything on-line. At the same time industrial organizations experience a pressure on their ability to train employees faster due to the increase in complexity. We argue that games are not yet mature enough to support this training challenge as stand alone efforts. But games can support the training and competence development in a synchronized setup with other means

Abelian homotopy Dijkgraaf-Witten theory

We construct a version of Dijkgraaf-Witten theory based on a compact abelian Lie group within the formalism of Turaev's homotopy quantum field theory. As an application we show that the 2+1-dimensional theory based on U(1) classifies lens spaces up to homotopy type.Comment: 23 pages, 1 figur

Co- and counter-helicity interaction between two adjacent laboratory prominences

The interaction between two side-by-side solar prominence-like plasmas has been studied using a four-electrode magnetized plasma source that can impose a wide variety of surface boundary conditions. When the source is arranged to create two prominences with the same helicity (co-helicity), it is observed that helicity transfer from one prominence to the other causes the receiving prominence to erupt sooner and faster than the transmitting prominence. When the source is arranged to create two prominences with opposite helicity (counter-helicity), it is observed that upon merging, prominences wrap around each other to form closely spaced, writhing turns of plasma. This is followed by appearance of a distinct bright region in the middle and order of magnitude higher emission of soft x rays. The four-electrode device has also been used to change the angle of the neutral line and so form more pronounced S-shapes

An improved 2.5 GHz electron pump: single-electron transport through shallow-etched point contacts driven by surface acoustic waves

We present an experimental study of a 2.5 GHz electron pump based on the quantized acoustoelectric current driven by surface acoustic waves (SAWs) through a shallow-etched point contact in a GaAs/AlGaAs heterostructure. At low temperatures and with an additional counter-propagating SAW beam, up to n = 20 current plateaus at I=nef could be resolved, where n is an integer, e the electron charge, and f the SAW frequency. In the best case the accuracy of the first plateau at 0.40 nA was estimated to be dI/I = +/- 25 ppm over 0.25 mV in gate voltage, which is better than previous results.Comment: 11 pages, 4 figure

Self-Diffusion in 2D Dusty Plasma Liquids: Numerical Simulation Results

We perform Brownian dynamics simulations for studying the self-diffusion in two-dimensional (2D) dusty plasma liquids, in terms of both mean-square displacement and velocity autocorrelation function (VAF). Super-diffusion of charged dust particles has been observed to be most significant at infinitely small damping rate $\gamma$ for intermediate coupling strength, where the long-time asymptotic behavior of VAF is found to be the product of $t^{-1}$ and $\exp{(-\gamma t)}$. The former represents the prediction of early theories in 2D simple liquids and the latter the VAF of a free Brownian particle. This leads to a smooth transition from super-diffusion to normal diffusion, and then to sub-diffusion with an increase of the damping rate. These results well explain the seemingly contradictory scattered in recent classical molecular dynamics simulations and experiments of dusty plasmas.Comment: 10 pages 5 figures, accepted by PR

Effect of feeding fermentable fibrerich feedstuffs on meat quality with emphasis on chemical and sensory boar taint in entire male and female pigs

Skatole, androstenone and other compounds such as indole cause boar taint in entire male pork. However, female pigs also produce skatole and indole. The purpose of this experiment was to minimise boar taint and increase overall impression of sensory quality by feeding entire male and female pigs with fibrerich feedstuffs. The pigs have been fed three organic diets for either 1 or 2 weeks prior to slaughter of which two diets contained different fermentable fibre-rich feedstuffs – 10–13.3% dried chicory roots or 25% blue lupines. These two treatments were compared with pigs fed with an organic control diet for either 1 or 2 weeks prior to slaughter. Lupines significantly reduced skatole in blood and backfat for both genders after 1 week. Moreover, lupines showed negative impact on growth rate and feed conversion whilst chicory showed no significant differences in this respect. However, the indole concentration was significantly lower in chicory than lupine fed pigs. From a sensory perspective, chicory and lupine feeding reduced boar taint since odour and flavour of manure related to skatole and urine associated to androstenone were minimised. The level of boar taint in the entire male pigs was most effectively reduced after 14 days by both fibre-rich feeds while lupine had the largest influence on ‘‘boar” taint reduction in female pigs

Peripherality of breakup reactions

The sensitivity of elastic breakup to the interior of the projectile wave function is analyzed. Breakup calculations of loosely bound nuclei (8B and 11Be) are performed with two different descriptions of the projectile. The descriptions differ strongly in the interior of the wave function, but exhibit identical asymptotic properties, namely the same asymptotic normalization coefficient, and phase shifts. Breakup calculations are performed at intermediate energies (40-70 MeV/nucleon) on lead and carbon targets as well as at low energy (26 MeV) on a nickel target. No dependence on the projectile description is observed. This result confirms that breakup reactions are peripheral in the sense that they probe only the external part of the wave function. These measurements are thus not directly sensitive to the total normalization of the wave function, i.e. spectroscopic factor.Comment: Reviewed version accepted for publication in Phys. Rev. C; 1 new section (Sec. III E), 2 new figures (Figs. 3 and 5

Asymmetries in the CMB anisotropy field

We report on the results from two independent but complementary statistical analyses of the WMAP first-year data, based on the power spectrum and N-point correlation functions. We focus on large and intermediate scales (larger than about 3 degrees) and compare the observed data against Monte Carlo ensembles with WMAP-like properties. In both analyses, we measure the amplitudes of the large-scale fluctuations on opposing hemispheres and study the ratio of the two amplitudes. The power-spectrum analysis shows that this ratio for WMAP, as measured along the axis of maximum asymmetry, is high at the 95%-99% level (depending on the particular multipole range included). The axis of maximum asymmetry of the WMAP data is weakly dependent on the multipole range under consideration but tends to lie close to the ecliptic axis. In the N-point correlation function analysis we focus on the northern and southern hemispheres defined in ecliptic coordinates, and we find that the ratio of the large-scale fluctuation amplitudes is high at the 98%-99% level. Furthermore, the results are stable with respect to choice of Galactic cut and also with respect to frequency band. A similar asymmetry is found in the COBE-DMR map, and the axis of maximum asymmetry is close to the one found in the WMAP data.Comment: 6 pages, 5 figures; version to appear in ApJ, textual improvements, added reference